C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
195/1.2, 195/1.2
C12N 15/18 (2006.01) C07K 14/61 (2006.01) C07K 14/775 (2006.01) C12N 9/02 (2006.01) C12N 9/08 (2006.01) C12N 15/53 (2006.01) C12N 15/70 (2006.01) C12P 21/02 (2006.01)
Patent
CA 1309043
EXPRESSION PLASMIDS CONTAINING THE DEO PROMOTER, AND BACTERIAL HOSTS CONTAINING THE PLASMIDS Abstract of the Disclosure Plasmids are provided which upon introduction into a suitable Escherichia coli host render the host capable of effecting expression of DNA encoding a desired naturally- occurring polypeptide or polypeptide analog thereof under the control of the deo P1-P2 promoter. Further plasmids are also provided which thermoinducibly direct expression of eucaryotic genes under the control of a .lambda. PL promoter and a thermolabile repressor protein present on the same plasmids under the control of a deo P1 promoter. These plasmids have been inserted into various hosts, some of which contain DNA encoding the repressor protein. Such plasmids may be used to produce polypeptides such as human copper/zinc superoxide dismutase, hMnSOD-hGPX fusion polypeptides, human manganese superoxide dismutase, human growth hormone, bovine growth, porcine growth, human growth hormone-apolipoprotein E fused polypeptides, apolipoprotein E and various fibronectin domains.
574717
Gowling Lafleur Henderson Llp
Savient Pharmaceuticals Inc.
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