Expression profiling using microarrays

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/68 (2006.01) C12P 19/34 (2006.01) C40B 20/02 (2006.01) C40B 20/04 (2006.01) C40B 40/00 (2006.01) C40B 40/10 (2006.01) C40B 50/06 (2006.01)

Patent

CA 2536565

The invention provides novel compositions and methods for the analysis of gene expression (e.g., expression profiling) using microarray-based technology. In some embodiments of the invention, the novel methods use gene-specific as well as universal amplification primers during sample preparation, and the methods permit the simultaneous analysis of multiple samples on the same microarray. Furthermore, some embodiments of the invention incorporate barcode sequences into the amplified products, thereby permitting the use of generic arrays and generic labeled probes.

L'invention concerne de nouvelles compositions et des nouveaux procédés d'analyse de l'expression génique (par ex., profilage d'expression) qui reposent sur la technologie des microréseaux. Dans certains modes de réalisation de l'invention, ces nouveaux procédés font intervenir des amorces d'amplification spécifiques des gènes ainsi que des amorces universelles au cours de la préparation d'échantillons, ces procédés permettant également d'analyser simultanément plusieurs échantillons sur le même microréseau. Par ailleurs, certains modes de réalisation de l'invention appliquent des séquences de codes à barres aux produits amplifiés, ce qui permet d'utiliser des réseaux génériques et des sondes génériques marquées.

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