Expression system, components thereof and methods of use

Details Expression system, components thereof and methods of use Expression system, components thereof and methods of use

C - Chemistry, Metallurgy

12

N

C12N 15/78 (2006.01) C07K 14/21 (2006.01) C12N 5/10 (2006.01) C12N 15/31 (2006.01) C12N 15/63 (2006.01) C12P 21/00 (2006.01)

Patent

CA 2580515

Recently, the development of inducible expression systems has involved exploitation of the p-cym operon from Pseudomonas putida. Disclosed herein are novel expression systems and components thereof, which involve the development of CymR variants with reverse DNA binding activity, such that they exhibit increased affinity for DNA in a presence rather than an absence of an effector molecule such as cumene or an equivalent thereof. Also disclosed are the CymR variants, fusion proteins incorporating such variants, and their use in the control and expression of polynucleotides.

Récemment, le développement de systèmes d'expression inductibles a impliqué l'exploitation de l'opéron p-cym issu de Pseudomonas putida. Cette invention concerne de nouveaux systèmes d'expression et des composants de ces systèmes d'expression, qui impliquent le développement de variantes CymR présentant une activité de liaison inverse à l'ADN, de manière que leur affinité pour l'ADN soit accrue, en la présence plutôt qu'en l'absence d'une molécule effectrice telle que cumène ou un équivalent de cumène. Cette invention concerne également des variantes CymR, des protéines de fusion comportant ces variantes, et leur utilisation pour la régulation et l'expression de polynucléotides.

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