Expression system for producing apolipoprotein ai-m

Details Expression system for producing apolipoprotein ai-m Expression system for producing apolipoprotein ai-m

C - Chemistry, Metallurgy

12

N

C12N 15/70 (2006.01) C07K 14/775 (2006.01) C12N 1/20 (2006.01) C12N 1/21 (2006.01) C12N 15/12 (2006.01) C12N 15/71 (2006.01)

Patent

CA 2150928

The invention relates to an expression system giving high extracellular production of apolipoprotein AI-M (Milano) using E. coli and comprises a plasmid carrying an origin of replication, an inducible promoter sequence, a DNA sequence coding for a signal peptide, a DNA sequence coding for apolipoprotein AI-M, and a transcription terminator. The invention also relates to a method of producing apolipoprotein AI-M using the expression system.

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