Expression system for the b subunit of cholera toxin

Details Expression system for the b subunit of cholera toxin Expression system for the b subunit of cholera toxin

C - Chemistry, Metallurgy

12

N

C12N 15/67 (2006.01) C12N 15/68 (2006.01) C12N 15/74 (2006.01)

Patent

CA 2543548

The present invention provides an expression system for obtaining improved yield of the B subunit of a cholera toxin (CTB) wherein the expression system comprises a Vibrio cholerae host cell lacking the functionality of a thyA gene; and an expression vector comprising a functional thyA gene and a CTB gene which is substantially free of the flanking sequences immediately contiguous by the 5~ and 3~ end of the CTB gene in the naturally occurring genome of the host cell from which the CTB gene is derived. The present invention also provides a method of producing CTB, and an isolated nucleic acid construct that is used as an expression vector in the expression system.

L'invention concerne un système d'expression pour produire une sous-unité B d'une toxine du choléra (CTB), comprenant une cellule hôte Vibrio cholerae ne présentant pas la fonctionnalité d'un gène thyA ; un vecteur d'expression comprenant un gène thyA et un gène de la CTB qui est sensiblement exempt des séquences flanquantes immédiatement contiguës aux extrémités 5' et 3' du gène de la CTB dans le génome apparaissant naturellement de la cellule hôte, à partir de laquelle le gène de la CTB est dérivé. L'invention concerne également un procédé de production de la CTB, et une construction d'acide nucléique isolée utilisée comme vecteur d'expression dans le système d'expression.

Affiliated with

Also associated with

Rating

Expression system for the b subunit of cholera toxin does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Expression system for the b subunit of cholera toxin, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Expression system for the b subunit of cholera toxin will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFCA-PAI-O-1909415