Expression system which can be regulated

Details Expression system which can be regulated Expression system which can be regulated

C - Chemistry, Metallurgy

12

N

C12N 15/74 (2006.01) C07K 14/26 (2006.01) C12N 1/21 (2006.01) C12N 9/10 (2006.01) C12N 15/11 (2006.01) C12N 15/31 (2006.01) C12N 15/70 (2006.01)

Patent

CA 2171668

An expression system can be regulated by acetate, pH and oxygen, which expression system includes a trans-acting regulator protein and a promoter which can be activated by this protein. Any desired structural genes are maximally expressed under the control of the expression system at an oxygen partial pressure, Po2, of 0-5% and a pH of 6.0-6.5, and in the presence of acetate at a concentration of 40-60 mM. There is also a process for preparing this expression system, and a process for using this expression system.

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