Expression systems

Details Expression systems Expression systems

C - Chemistry, Metallurgy

12

N

C12N 15/85 (2006.01) C07K 14/47 (2006.01) C07K 14/61 (2006.01) C07K 14/715 (2006.01) C07K 14/72 (2006.01) C07K 14/765 (2006.01) C07K 14/805 (2006.01) C12N 9/20 (2006.01) C12N 15/64 (2006.01) C12P 21/08 (2006.01)

Patent

CA 2058280

ABSTRACT Expression systems which comprise a mammalian host, such as erythroid cells, transformed with a vector which comprises a promoter, a DNA sequence which codes for a desired polypeptide and a dominant control region. The vectors and methods for preparing polypeptides using the expression systems are also described. In a preferred embodiment, the vector contains cDNA for the desired polypeptide and a sequence which is capable of stabilising mRNA produced from the cDNA. The expression systems are particularly efficient and are capable of secreting the desired polypeptide.

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