Expression systems for amidating enzyme

C - Chemistry – Metallurgy – 12 – N

Patent

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195/1.2, 195/1.3

C12N 15/60 (2006.01) C12N 1/00 (2006.01) C12N 9/02 (2006.01) C12N 15/67 (2006.01)

Patent

CA 2009306

Alpha-amidating enzyme is produced by recombinant DNA techniques recoverable in high yields and at high purity. Both eukaryotic and prokaryotic expression vectors are provided having a transcriptional promoter followed downstream by a DNA sequence which encodes amidating enzyme. The vector selected is one capable of directing the expression of polypeptides in the host selected, and preferred hosts are transected with the described vectors.

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