Expression systems for preparation of polypeptides in...

Details Expression systems for preparation of polypeptides in... Expression systems for preparation of polypeptides in...

C - Chemistry, Metallurgy

12

N

195/1.2, 195/1.3

C12N 15/70 (2006.01) C12N 1/21 (2006.01)

Patent

CA 1335357

Expression cassettes for enhanced expression and production of a polypeptide of interest in prokaryotic cells are provided. The expression cassettes provide for production of the polypeptide of interest so that such polypeptide can either be secreted from the host cell in an active conformation or conveniently processed and renatured to a functional state. Preferably, the polypeptide of interest is expressed as a fusion protein, particularly fused to a leader sequence from a highly expressed bacterial or bacteriophage gene. The polypeptide of interest may subsequently be cleaved from the leader sequence and refolded, or used as a fusion protein.

581704

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