Expression systems using mammalian .beta.-actin promoter

Details Expression systems using mammalian .beta.-actin promoter Expression systems using mammalian .beta.-actin promoter

C - Chemistry, Metallurgy

12

N

C12N 15/11 (2006.01) A01K 67/027 (2006.01) C12N 15/10 (2006.01) C12N 15/85 (2006.01) C12P 21/02 (2006.01)

Patent

CA 2548185

As the results of a comparison and discussion on the activities of promoters prepared by combining various gene promoters and enhancers, it is found out that a hybrid promoter comprising a combination of CMV enhancer with a mammalian .beta.-actin promoter or a combination of the posttranscriptional regulatory element of the genomic sequence of Woodchuck Hepatitis Virus (WPRE) with a mammalian .beta.-actin promoter is superior in potency to the existing promoters. It is also found out that the activity of this .beta.-actin promoter is enhanced by the simultaneous expression of an oncogene product Ras which is a transactivator.

La comparaison et l'examen des activités de promoteurs obtenus par combinaison de divers promoteurs et accentuateurs de gène ont fait apparaître qu'un promoteur hybride contenant une combinaison d'accentuateur CMV et d'un promoteur de beta -actine de mammifère ou une combinaison de l'élément régulateur post-transcriptionnel de la séquence génomique du virus de l'hépatite de la marmotte et d'un promoteur de beta -actine de mammifère présentait une puissance supérieure à celle des promoteurs existants. On a également constaté que l'activité de ce promoteur de beta -actine était accentuée par l'expression simultanée d'un produit oncogène Ras, qui est un transactivateur.

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