Expression using fused genes providing for protein product

Details Expression using fused genes providing for protein product Expression using fused genes providing for protein product

C - Chemistry, Metallurgy

12

N

530/13, 195/1.23

C12N 15/00 (2006.01) C07K 14/16 (2006.01) C07K 14/62 (2006.01) C07K 14/65 (2006.01) C12N 9/02 (2006.01) C12N 15/81 (2006.01) C12P 21/02 (2006.01)

Patent

CA 1260858

36 IMPROVED EXPRESSION USING FUSED GENES PROVIDING FOR PROTEIN PRODUCT ABSTRACT OF THE DISCLOSURE Novel methods and compositions are provided for enhanced yield of heterologous proteins in fungi. The method and compositions involve employing fusion sequences involving a sequence encoding a heterologous product produced in relatively large amount as a stable polypeptide in the host fused to a second sequence in open reading frame with the prior sequence coding for a different heterologous polypeptide, where the two poly- peptides are joined by a selectively cleavable linkage. In particular, a sequence coding for superoxide dismutase is joined to another polypeptide of interest at either terminus of the superoxide dismutase in a yeast expression vector under transcriptional control of an active promoter and the vector introduced into a yeast host and the host grown. High yields of the fusion product are obtained in this manner, where the fusion product can be selectively cleaved so as to produce both the superoxide dismutase and the other polypeptide in high yield. The S. cerevisiae strains 2150-2-3 (pYASIl) and AB110 (pYLUIGF2-14) were deposited at the A.T.C.C. on February 27, 1985 and March 19, 1986, respectively; and given accession nos. 20745 and 20796, respectively.

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