Expression vector for use in a one-step purification protocol

Details Expression vector for use in a one-step purification protocol Expression vector for use in a one-step purification protocol

C - Chemistry, Metallurgy

12

N

C12N 15/62 (2006.01) C07K 14/445 (2006.01) C12N 15/30 (2006.01) C12N 15/79 (2006.01)

Patent

CA 2333602

The invention relates to an expression vector that can be used with a single- step purification procedure. The recombinant protein is produced as a fusion protein with a lectin, in particular a discoidin, as a purification tag. The fusion protein can be purified on a sepharose 4B chromatography column, using galactose as eluent.

L'invention porte sur un vecteur d'expression qui peut être utilisé dans un processus de purification en une étape. La protéine de recombinaison est produite sous forme d'une protéine de fusion avec une lectine, notamment une discoïdine, comme étiquette de purification. La protéine de fusion peut être purifiée sur une colonne de chromatographie Sepharose 4B au moyen d'une galactose comme éluant.

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