Expression vectors

Details Expression vectors Expression vectors

C - Chemistry, Metallurgy

12

N

195/1.18, 195/1.

C12N 15/11 (2006.01) C07K 14/735 (2006.01) C12N 1/21 (2006.01) C12N 5/10 (2006.01) C12N 9/72 (2006.01) C12N 15/67 (2006.01) C12N 15/85 (2006.01) C12P 21/00 (2006.01)

Patent

CA 1310601

Expression vectors Abstract of the disclosure Recombinant DNA molecules comprising a series of functional elements in the following order: (a) an enhancer/promoter unit linked opera- tively to (b) a DNA segment which can be transcribed into a mRNA having coding capacity for a desired protein product and comprising signals for polyadenylation and for efficient transportation to the cytoplasm, followed by a DNA segment (c) consisting a promoter unit (c1) operatively linked to a DNA subsegment (c2) which can be trans- cribed into the mRNA of a selection marker gene, wherein said promoter unit (c1) lacks a functional enhancer hut is capable of being stimulated by the enhancer located in (a) due to the absence of an enhancer stop DNA sequence between (a) and (cl), are provided. The specific arrangement of the functional elements assures that a high proportion of transfected eukaryotic cells stably express the heterologous gene even when said DNA is applied to said eukaryotic cells as circular DNA.

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