Expression vectors and methods

Details Expression vectors and methods Expression vectors and methods

C - Chemistry, Metallurgy

12

N

C12N 15/12 (2006.01) C07K 14/435 (2006.01) C07K 14/71 (2006.01) C07K 16/42 (2006.01) C12N 15/10 (2006.01) C12N 15/62 (2006.01) C12N 15/65 (2006.01) C12N 15/69 (2006.01) C12N 15/85 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2385102

Vectors and methods for efficient isolation of recombinant cells expressing high levels of a desired protein are provided. The vectors comprise an amplifiable selectable gene, a fluorescent protein gene, and a gene encoding a desired product in a manner that optimizes transcriptional and translational linkage.

La présente invention concerne des vecteurs et des procédés destinés à une isoler efficacement des cellules recombinantes exprimant des niveaux élevés d'une protéine recherchée. Les vecteurs comprennent un gène amplifiable que l'on peut sélectionner, un gène de protéine fluorescente et un gène codant pour un produit recherché d'une manière qui optimise un couplage de transcription et de translation.

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