Expression vectors and uses thereof

Details Expression vectors and uses thereof Expression vectors and uses thereof

C - Chemistry, Metallurgy

12

N

C12N 15/85 (2006.01) C07K 14/15 (2006.01) C12N 15/867 (2006.01)

Patent

CA 2426647

The invention includes viral vectors (e.g., retrovial vectors, e.g., replication deficient retrovial vectors), libraries comprising such vectors, retroviral particles produced by such vectors, retroviral packaging cell lines for production of these particles, integrated proviral sequences derived from the retroviral particles, circularized provirus sequences and mammalian cells upon which the provirus has been introduced. The invention also includes methods of using such sequence, vectors, particles and cells.

L'invention concerne des vecteurs viraux (par exemple, des vecteurs rétroviraux, par exemple des vecteurs rétroviraux à réplication déficiente), des bibliothèques comprenant de tels vecteurs, des particules rétrovirales produites par de tels vecteurs, des lignes cellulaires d'enrobage rétroviral pour produire ces particules, des séquences provirales intégrées dérivées des particules rétrovirales, des séquences de provirus circularisé et des cellules mammifères sur lesquelles on a introduit le provirus. L'invention concerne également des procédés d'utilisation de ces séquences, vecteurs, particules et cellules.

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