C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/00 (2006.01) C07H 21/04 (2006.01) C07K 14/00 (2006.01) C07K 14/705 (2006.01) C12N 15/63 (2006.01) C12N 15/85 (2006.01)
Patent
CA 2344699
A specific locus (hot spot) for recombinant gene expression has been identified in the genome of Chinese hamster ovary cells. A DNA vector containing the hot spot causes high levels of recombinant gene expression following transfection and stable integration. The selection and cloning of the specific locus and the expression of recombinant genes is disclosed, as are the DNA vectors and the host cells.
L'invention concerne un locus (point chaud) identifié dans le génome de cellules ovariennes de hamster chinois, ce locus permettant d'améliorer l'expression de gènes recombinants. En effet, la présence d'un vecteur d'ADN contenant le point chaud a pour effet d'améliorer grandement l'expression de gènes recombinants suite à la transfection et à l'intégration stable des cellules. L'invention porte également la sélection et le clonage de ce locus spécifique, l'expression de gènes recombinants ainsi que les vecteurs d'ADN et les cellules hôtes susmentionnés.
Koduri Kanakaraju
Miller John T.
Thammana Pallaiah
Bristol-Myers Squibb Company
Gowling Lafleur Henderson Llp
LandOfFree
Expression vectors containing hot spot for increased... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Expression vectors containing hot spot for increased..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Expression vectors containing hot spot for increased... will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1496951