C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/62 (2006.01) C07K 14/16 (2006.01) C07K 16/28 (2006.01) C07K 16/30 (2006.01) C07K 16/46 (2006.01) C07K 19/00 (2006.01) C12N 5/10 (2006.01) C12N 15/13 (2006.01) C12P 21/02 (2006.01) G01N 33/563 (2006.01) G01N 33/569 (2006.01) G01N 33/574 (2006.01) A61K 38/00 (2006.01)
Patent
CA 2114353
The present invention provides an expression vector encoding monospecific or bispecific fusion protein. In one embodiment the expression vector encodes a monospecific fusion protein, which vector comprises a recombinant monospecific single chain cassette comprising a DNA sequence encoding a first binding domain capable of binding a cell surface antigen. In another embodiment the expression vector encodes a bispecific fusion protein, which vector comprises a recombinant bispecific single chain cassette comprising a DNA sequence encoding a first binding domain capable of binding a cell surface antigen and a DNA sequence encoding a second binding domain capable of binding a cell surface antigen, each domain capable of binding a different antigen. The present invention also provides a method for producing a biologically active monospecific or bispecific fusion protein in a mammalian cell. This method comprises: (a) transfecting the mammalian cell with the recombinant expression vector of the invention; (b) culturing the mammalian cell so transfected in step (a); and (c) recovering the biologically active bispecific fusion protein so as produced by the cultured mammalian cell.
Bajorath Jurgen
Fell H. Perry
Gilliland Lisa K.
Hayden Martha S.
Ledbetter Jeffrey A.
Bristol-Myers Squibb Company
Cassan Maclean
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