C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
195/1.16, 530/3.
C12N 15/00 (2006.01) C07K 14/61 (2006.01) C12N 15/73 (2006.01) C12P 21/02 (2006.01) A61K 38/00 (2006.01)
Patent
CA 1254527
EXPRESSION VECTORS FOR ENHANCED PRODUCTION OF POLYPEPTIDES, PLASMIDS CONTAINING THE VECTORS, HOSTS CONTAINING THE PLASMIDS, PRODUCTS MANUFACTURED THEREBY AND RELATED METHODS ABSTRACT OF THE DISCLOSURE An improved vector upon introduction into a suitable bac- terial host containing the thermolabile repressor CI ren- ders the host cell capable, upon increasing the temperature of the host cell to a temperature at which the repressor is destroyed, of effecting expression of a desired gene in- serted into the vector and production of polypeptide en- coded by the gene. The vector is a double-stranded DNA molecule which includes in 5' to 3' order the following: a DNA sequence which contains the promoter and operator PLOL from lambda bacteriophage; the N utilization site for binding antiterminator N protein produced by the host cell; a DNA sequence which contains a ribosomal binding site for rendering the mRNA of the desired gene capable of binding to ribosomes within the host cell; an ATG initiation codon or a DNA sequence which is converted into an ATG initiation codon upon insertion of the desired gene into the vector; a restriction enzyme site for inserting the desired gene into the vector in phase with the ATG initiation codon; and additionally a DNA sequence which contains an origin of replication from a bacterial plasmid capable of autonomous replication in the host cell and a DNA sequence which contains a gene associated with a selectable or identifi- able trait which is manifested when the vector is present in the host cell.
458833
Aviv Haim
Gorecki Marian
Levanon Avigdor
Oppenheim Amos
Vogel Tikva
Gowling Lafleur Henderson Llp
Savient Pharmaceuticals Inc.
LandOfFree
Expression vectors for enhanced production of polypeptides,... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Expression vectors for enhanced production of polypeptides,..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Expression vectors for enhanced production of polypeptides,... will most certainly appreciate the feedback.
Profile ID: LFCA-PAI-O-1334075