C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
195/120, 195/124
C12N 15/00 (2006.01) C07H 21/04 (2006.01) C07K 14/61 (2006.01) C12N 5/00 (2006.01) C12N 15/73 (2006.01)
Patent
CA 1277263
Abstract The present invention provides a transcrip- tional and translational activating sequence derived from the lambda pL transcriptional activating sequence and the E. coli lpp translational activating sequence. The activating sequence has been cloned into recombinant DNA expression vectors into which DNA sequences encoding functional polypeptides can be readily inserted and expressed. The activating sequence of the present invention has been shown to drive high-level expression of a bovine growth hormone derivative and a human growth hormone derivative in E. coli. Preferred expression vectors of the present invention also comprise the cI857 temperature-sensitive lambda pL repressor gene, a rop- derivative of the plasmid pBR322 replicon, and a tetracycline resistance-conferring gene.
516373
Eli Lilly And Company
Gowling Lafleur Henderson Llp
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