B - Operations – Transporting – 01 – D
Patent
B - Operations, Transporting
01
D
B01D 15/08 (2006.01) C07K 1/18 (2006.01) G01N 30/60 (2006.01)
Patent
CA 2525880
The invention provides novel methods for the separation of charged molecules such a proteins according to their isoelectric points (pI's) and includes the systems and buffering compositions employed for isolating charged molecules. The invention further provides for modifications to the above described chromatographic methods that enable the separation of charged molecules exhibiting virtually identical pl's by shifting both the buffer's pKa and the pl's of the eluted charged molecules while they are traversing the ion exchange column.
L'invention se rapporte à des nouveaux procédés permettant la séparation de molécules chargées telles que des protéines en fonction de leurs points isoélectriques (pI) et elle concerne les systèmes et les compositions de tamponnage utilisés pour isoler les molécules chargées. L'invention se rapporte également à des modifications des procédés chromatographiques décrits ci-dessus qui permettent la séparation de molécules chargées présentant des points isoélectriques sensiblement identiques par décalage à la fois du pKa et du pI du tampon des molécules chargées eluées lorsque celles-ci traversent la colonne d'échange d'ions.
Hirsh Allen G.
Tsonev Latchezar I.
Cryobiophysica Inc.
Gowling Lafleur Henderson Llp
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