Extracellular matrix coated surface for culturing cells

Details Extracellular matrix coated surface for culturing cells Extracellular matrix coated surface for culturing cells

C - Chemistry, Metallurgy

12

M

C12M 3/00 (2006.01) C12N 5/02 (2006.01) C12N 5/06 (2006.01) C12N 5/08 (2006.01)

Patent

CA 2588547

A cell culture product is provided for propagating embryonic stem cells, and maintaining their self-renewal and pluripotency characteristics for extended periods of time in culture. The cell culturing product includes a substrate; and a coating thereon deposited from a coating solution. The coating solution includes a mixture of extracellular matrix proteins and an aqueous solvent, wherein the total protein concentration in the coating solution is about 10 µg/ml to about 1mg/ml.

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