Extracellular matrix compositions

Details Extracellular matrix compositions Extracellular matrix compositions

A - Human Necessities

61

K

A61K 38/39 (2006.01) C12N 5/07 (2010.01) C12N 5/073 (2010.01) A61K 8/65 (2006.01) A61K 38/17 (2006.01) A61L 15/32 (2006.01) A61L 27/34 (2006.01) A61L 29/08 (2006.01) A61L 31/10 (2006.01) A61P 41/00 (2006.01) A61Q 7/00 (2006.01) A61Q 19/08 (2006.01) C12P 21/00 (2006.01) C12P 21/02 (2006.01) C07K 14/475 (2006.01) C07K 14/78 (2006.01)

Patent

CA 2714387

The present invention is directed to a method of producing compositions including embryonic proteins. The method includes culturing cells under hypoxic conditions on a biocompatible two- or three-dimensional surface in vitro. The culturing method produces both soluble and non-soluble fractions, which may be used separately or in combination to obtain physiologically acceptable compositions useful in a variety of medical and therapeutic applications.

La présente invention concerne un procédé de production de compositions qui comprend des protéines embryonnaires. Le procédé comprend la mise en culture de cellules dans des conditions hypoxiques sur une surface biocompatible en deux ou trois dimensions in vitro. Le procédé de culture produit des fractions, à la fois solubles et non solubles, qui peuvent être utilisées séparément ou en combinaison pour obtenir des compositions physiologiquement acceptables qui s'utilisent dans une variété d'applications médicales et thérapeutiques.

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