Extracorporeal cell culture and transplantation kits

Details Extracorporeal cell culture and transplantation kits Extracorporeal cell culture and transplantation kits

C - Chemistry, Metallurgy

12

M

C12M 3/00 (2006.01) C12N 5/02 (2006.01) C12Q 1/00 (2006.01) C12P 21/08 (2006.01) C12N 5/08 (2006.01) C12N 5/06 (2006.01)

Patent

CA 2184498

An extracorporeal stem cell culture and transplantation kit comprising means for selecting cells having a desired phenotype in a cell mixture obtained from a human; means for isolating the selected cells from the mixture; means for incubating the isolated cells; a composition comprising an effective amount of a cellular expansion factor, wherein the expansion factor is selected from the group consisting of: GM-CSF, G-CSF, IL-1, IL-3, IL-6, TPO, EPO, flt3-ligand, SF and a GM-CSF/IL-3 fusion protein; and cellular growth medium.

On décrit une culture extra-corporelle de cellules souches, et une trousse de transplantation qui contient un dispositif permettant de choisir des cellules d'un phénotype souhaité dans un mélange de cellules provenant d'un être humain, un dispositif permettant d'isoler les cellules choisies de ce mélange, un dispositif permettant de faire incuber les cellules isolées, et une composition comprenant une quantité efficace d'un facteur d'expansion cellulaire choisi dans le groupe consistant en: GM-CSF (facteur de stimulation de colonies de granulocytes et macrophages), G-CSF (concernant les seuls macrophages), interleukines 1, 3 et 6, thrombopoiétine, érythropoiétine, ligands du récepteur flt3, facteur de croissance de cellules souches et une protéine de fusion GM-CSF/IL3, ainsi qu'en un milieu de croissance cellulaire.

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