Extraction and utilisation of vntr alleles

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01)

Patent

CA 2283651

The invention presented is a novel method for the extraction of VNTR alleles and for the concomitant detection of polymorphic markers for inherited traits at multiple loci by simultaneous comparison of complex genomes from multiple individuals. The product is designated a Total Representation of Alleles that are Informative for a Trait (TRAIT). These alleles may be used directly as genetic markers or may be used as vehicles to facilitate precise localisation of sequence variations responsible.

La présente invention concerne une nouvelle méthode d'extraction d'allèles VNTR et de dépistage concomitant de marqueurs polymorphes pouvant révéler des caractéristique héréditaires dans de multiples loci, qui s'effectue par comparaison simultanée de génomes complexes provenant de multiples individus. Le résultat est appelé "totale représentation d'allèles informant sur un trait caractéristique" (TRAIT). Lesdits allèles peuvent être utilisés directement comme marqueurs génétiques, ou comme vecteurs permettant de localiser facilement l'emplacement précis d'un responsable de variations de séquence.

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