Extraction methods for preparing thromboplastin reagents

Details Extraction methods for preparing thromboplastin reagents Extraction methods for preparing thromboplastin reagents

C - Chemistry, Metallurgy

12

N

195/39.1, 167/10

C12N 9/48 (2006.01) C07K 14/745 (2006.01) C12N 9/64 (2006.01) C12Q 1/56 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2002208

The Prothrombin Time (PT) is used as a screening test for blood coagulation factor deficiencies and for monitoring oral anti-coagulant therapy using coumadin. Thromboplastin reagents activate the extrinsic pathway of coagulation and are the basis for the Prothrombin Time (PT) test. This invention describes the use of barium sulfate and chaotropic agents, and nonionic detergents, for the extraction of sensitive thromboplastin reagents from tissue. Extraction with sodium thiocyanate alone also greatly enhances thromboplastin sensitivity. This invention should be useful for all thromboplastins and will improve their sensitivity for all PT-based tests and specific assays.

Le temps de prothombine (TP) sert à dépister les carences en facteur de coagulation du sang et à surveiller la thérapie orale anti-coagulant par la coumadine. Les thromboplastines activent la voie extrinsèque de coagulation et constituent la base de l'essai du TP. L'invention décrit l'emploi de sulfate de baryum et d'agents chaotropiques, ainsi que de détergents non ioniques, pour l'extraction de thromboplastines sensibles à partir des tissus. L'extraction avec le thiocyanate de sodium seul augmente aussi considérablement la sensibilité de la thromboplastine. L'invention devrait être utile pour toutes les thromboplastines et améliorera leur sensibilité pour tous les essais de type TP et les épreuves spécifiques.

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