Extraction of dna from biological samples

Details Extraction of dna from biological samples Extraction of dna from biological samples

C - Chemistry, Metallurgy

07

H

C07H 21/04 (2006.01) C07H 1/08 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2501308

Methods and compositions are provided for extracting DNA from tissues, hair, teeth, bone, plant material, solid matrices and other samples from which DNA extraction is generally regarded as being difficult. DNA may quickly be extracted from samples, for example, in just 5 minutes to 2 hours, without enzymatic digestion or the use of toxic organic chemicals such as phenol, chloroform, guanidine thiocyanate or 2-mercaptoethanol.

L'invention concerne des procédés et des compositions utilisés pour extraire de l'ADN contenu dans des tissus, des cheveux, des dents, des os, des matériaux végétaux, des matrices solides et d'autres échantillons à partir desquels l'extraction d'ADN est considérée de manière générale comme étant difficile. L'ADN peut ainsi être extrait rapidement d'échantillons, en l'espace, par exemple, de 5 minutes à 2 heures, sans digestion enzymatique ou sans utilisation de produits chimiques toxiques tels que le phénol, le chloroforme, le thiocyanate de guanidine ou le 2-mercapto-éthanol.

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