Extraction of nucleic acids using small diameter...

Details Extraction of nucleic acids using small diameter... Extraction of nucleic acids using small diameter...

C - Chemistry, Metallurgy

07

H

C07H 21/00 (2006.01) B03C 1/00 (2006.01) C07H 1/06 (2006.01) C12N 15/10 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2503206

A method includes creating a mixture in a container, the mixture including a sample containing at least one molecule of nucleic acid, at least one magnetically- responsive particle, and a remainder; and providing the mixture with a pH of less than about 7.0, thereby altering the surface charge properties of the at least one magnetically-responsive particle. The alteration in the surface change properties of the at least one magnetically-responsive particle causes the at least one molecule of nucleic acid to become non-specifically bound to the at least one magnetically-responsive particle to form a complex. Preferably, the at least one magnetically-responsive particle is uncoated, untreated, lacks surface modification, and has a diameter of about 0.1 µ to about 1.0 µ.

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