Extraction of polysaccharides from vegetable and microbial...

C - Chemistry – Metallurgy – 08 – B

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C08B 37/00 (2006.01) C07H 3/06 (2006.01)

Patent

CA 2454025

Useful polysaccharides, such as .beta.-1,3-glucans, from a biological raw material can be solubilised and/or isolated by treating the raw material with an oxidising agent that leads to oxidation of primary hydroxyl groups in the glucan. The oxidising agent is preferably a catalytic amount of a nitroxyl compound in the presence of a re-oxidising agent such as hypochlorite or an oxidative enzyme with oxygen or hydrogen peroxide. The polysaccharide retains its useful properties during this treatment and is, moreover, more readily available. If desired, protein material from the raw material can also be utilised.

Des polysaccharides utiles tels que des .beta.-1,3-glucans issus d'une matière première végétale peuvent être solubilisés et/ou isolés en traitant la matière première avec un agent d'oxydation qui entraîne l'oxydation des groupes hydroxyle primaires dans le glucan. L'agent d'oxydation est de préférence une quantité catalytique d'un composé nitroxyle en présence d'un agent de réoxydation tel qu'un hypochlorite ou une enzyme oxydante avec l'oxygène ou le peroxyde d'hydrogène. Le polysaccharide conserve ses propriétés utiles pendant ce traitement et est, en outre, plus facilement disponible. Si on le souhaite, on peut également utiliser la matière protéinique de la matière première.

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