Extraction process for a pharmaceutical product

C - Chemistry – Metallurgy – 07 – K

Patent

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C07K 1/14 (2006.01) C07K 14/435 (2006.01)

Patent

CA 2525488

A process for isolating a soluble, native collagen from a marine invertebrate, comprising the steps of: 1) treating a collagen-containing portion of the marine invertebrate with a weak acid solution in order to solubilise native collagen fibrils; 2) centrifuging the resultant slurry to remove tissue particulates; 3) adjusting the pH of the supernatant in order to precipitate collagen by addition of a base; 4) collecting the precipitated collagen; 5) resuspending the precipitated collagen; and performing buffer exchange against water using an 15 ultrafiltration membrane.

L'invention concerne un procédé d'extraction d'un collagène natif soluble d'un invertébré marin. Le procédé consiste à: 1) traiter une partie de l'invertébré marin contenant du collagène avec une solution faiblement acide aux fins de solubiliser les fibrilles du collagène natif; 2) centrifuger la boue résultante pour en retirer les particules tissulaires; 3) régler le pH du surnageant afin de précipiter le collagène par adjonction d'une base; 4) recueillir le collagène précipité; 5) remettre en suspension le collagène précipité; et procéder à un échange tampon contre l'eau au moyen d'une membrane d'ultrafiltration.

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