F(ab')2 immunoglobulin fragments for assay of antigen

G - Physics – 01 – N

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G01N 33/68 (2006.01) A61K 39/44 (2006.01) G01N 33/563 (2006.01)

Patent

CA 1118345

ABSTRACT IMMUNOASSAYS USING F(ab')2 FRAGMENTS Immunoassays for antigens or haptens are effec- ted using, instead of an immunoglobulin antibody, the F(ab')2 fragments thereof. In this way, interference from endogenous RF and C1q in the fluid under assay is avoided without the necessity of pre-treating the fluid to remove or inactivate the RF or C1q. The assays may be of various types including latex agglutination and competitive binding assays. A reagent for such use comprising F(ab')2 fragments insolubilised on a water- insoluble substrate, especially a particulate substrate, is described.

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