Facs assisted methods for introducing individual chromosomes...

Details Facs assisted methods for introducing individual chromosomes... Facs assisted methods for introducing individual chromosomes...

C - Chemistry, Metallurgy

12

N

C12N 15/87 (2006.01) C12N 5/10 (2006.01)

Patent

CA 2353559

The present invention provides methods and apparatus for the delivery of at least one chromosome into a cell. Invention methods and apparatus employ FACS or MACS technology for rapidly processing cells and for confirming the introduction of chromosome(s) into a cell. The introduction of the chromosome(s) into the cell is mediated by one or more of a laser, a linear accelerator or electrically induced fusion of a cell and encapsulated chromosome(s). Invention methods provide for the rapid and reliable processing associated with FACS and MACS technology to process thousands of cells a minute, thereby enabling large scale gene transfer.

La présente invention concerne des procédés et un appareil servant à acheminer au moins un chromosome à l'intérieur d'une cellule. Les procédés et l'appareil de l'invention mettent en oeuvre une technique FACS ou MACS pour traiter rapidement des cellules et confirmer l'introduction d'un ou plusieurs chromosomes à l'intérieur d'une cellule. L'introduction d'un ou plusieurs chromosomes à l'intérieur d'une cellule s'effectue par laser et/ou accélérateur linéaire et/ou fusion par électricité d'une cellule et d'un ou plusieurs chromosomes encapsulés. Les procédés de l'invention associés à une technique FACS ou MACS permettent un traitement rapide et fiable de milliers de cellules à la minute, ce qui autorise un transfert de gènes à grande échelle.

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