Factor to grow tissue ex vivo

Details Factor to grow tissue ex vivo Factor to grow tissue ex vivo

C - Chemistry, Metallurgy

12

N

C12N 11/02 (2006.01) A61K 31/70 (2006.01) A61K 35/12 (2006.01) A61K 35/36 (2006.01) A61K 38/17 (2006.01) A61L 15/32 (2006.01) A61L 17/00 (2006.01) A61L 27/00 (2006.01) A61L 27/60 (2006.01) C07K 14/47 (2006.01) C07K 14/78 (2006.01) C12M 3/00 (2006.01) C12N 5/00 (2006.01) C12N 15/12 (2006.01) A61K 38/00 (2006.01) C12N 5/06 (2006.01) C12N 5/08 (2006.01)

Patent

CA 2194057

A method of producing recombinant transforming growth factor .beta.-induced H3 protein and the use of this protein to accelerate wound healing. H3 promoted adhesion of human dermal fibroblasts to tissue culture plastic. The protein is applied directly to a wound or is used to promote adhesion and spreading of dermal fibroblasts to a solid support such as a nylon mesh which is then applied to the wound. In addition, CHO cells expressing H3 inhibited tumor cell growth.

L'invention concerne un procédé de production d'une protéine H3 induite par le facteur transformant de croissance-.beta., et l'utilisation de cette protéine pour accélérer la cicatrisation de lésions. L'invention porte également sur l'adhésion induite par H3 de fibroblastes dermiques humains sur du plastique de culture tissulaire. La protéine est appliquée directement sur une lésion ou est utilisée pour favoriser l'adhésion et la prolifération de fibroblastes dermiques sur un support solide tel que du tulle de nylon qui est ensuite appliqué sur la lésion. De plus, l'invention porte sur des cellules CHO exprimant la croissance de cellules tumorales inhibées par H3.

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