Factor viii:ca chromogenic assay

G - Physics – 01 – N

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G01N 33/86 (2006.01) C12Q 1/56 (2006.01)

Patent

CA 2065370

A chromogenic assay for determination of blood coagulation Factor VIII:Ca, using an indicator of Factor Xa simultaneously as a mesure of Factor VIII:Ca concentration and as an inhibitor of Factor Xa. This technique can be applied to measure the concentration of an activating enzyme using the rate of conversion of the indicator molecule of the product of that enzyme as an indirect indicator of enzyme concentration.

Dosage chromogénique servant à déterminer le Facteur VIII:Ca de coagulation sanguine en utilisant un indicateur du Facteur Xa à la fois comme un élément de mesure de la concentration du Facteur VIII:Ca et comme un inhibiteur du Facteur Xa. Cette technique peut être employée pour mesurer la concentration d'une enzyme activante. Dans ce cas, le taux de conversion de la molécule indicatrice du produit de cette enzyme est utilisé comme indicateur indirect de la concentration de l'enzyme.

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