Factor viii separation

C - Chemistry – Metallurgy – 07 – K

Patent

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Details

C07K 14/755 (2006.01) B01D 57/02 (2006.01) B01D 61/00 (2006.01) B01D 61/42 (2006.01) C07K 1/26 (2006.01) G01N 27/447 (2006.01)

Patent

CA 2453388

Methods for isolating functionally active Factor VIII using a membrane-based separation system containing a separation membrane to create a first and second interstitial volume between at least two restriction membranes. One or more stabilizing agents are added to the sample and/or an interstitial volume. A solvent in the first interstitial volume maintains FVIII in a desired charge state. Applying a potential between the first and second interstitial volumes separates FVIII on one side of the separation membrane from unwanted molecules on the other side of the separation membrane. These methods may also be used as a substitute for one or more steps in a conventional purification scheme for the separation of native or recombinant FVIII.

L'invention concerne des procédés d'isolement du facteur VIII fonctionnellement actif à l'aide d'un système de séparation membranaire comportant une membrane de séparation servant à créer un premier et un second volume interstitiel entre au moins deux membranes de restriction. Un ou plusieurs agents de stabilisation sont ajoutés à l'échantillon et/ou au volume interstitiel. Un solvant présent dans le premier volume interstitiel maintient le FVIII dans l'état de charge souhaité. L'application d'un potentiel entre les premier et second volumes interstitiels isole le FVIII d'un côté de la membrane de séparation, à l'écart des molécules non désirées présentes de l'autre côté de la membrane de séparation. On peut également substituer ces procédés à une ou plusieurs opérations d'un processus de purification classique pour isoler le FVIII natif ou recombiné.

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