Family 6 cellulases with increased thermostability,...

Details Family 6 cellulases with increased thermostability,... Family 6 cellulases with increased thermostability,...

C - Chemistry, Metallurgy

12

N

C12N 15/56 (2006.01) C12N 9/42 (2006.01) C12N 15/80 (2006.01) C12N 15/81 (2006.01) C12P 19/14 (2006.01) C12S 3/04 (2006.01)

Patent

CA 2666741

Amino acid sequences derived from Family 6 cellulases are disclosed that comprise a proline residue at position 413, with reference to the amino acid numbering of SEQ ID NO: 1 Additionally, the amino acid sequences may comprise one or more modifications selected from the group comprising, a serine or threonine residue at position 231, a serine or threonine residue at position 305, and a glutamine or asparagine residue at position 410, with reference to the amino acid numbering of SEQ ID NO: 1, SEQ ID NO: 21, and SEQ ID NO: 22, wherein the cellulase exhibits one or more of increased thermostability, increased thermophilicity, increased alkalophilicity, or a combination thereof.

L'invention concerne des séquences d'acides aminés dérivées de cellulases de famille 6, comprenant un résidu proline à la position 413, en se référant à la numérotation d'acides aminés de la SEQ ID NO : 1. Les séquences d'acides aminés peuvent également comprendre une ou plusieurs modifications choisies dans le groupe comprenant un résidu sérine ou thréonine à la position 231, un résidu sérine ou thréonine à la position 305, et un résidu glutamine ou asparagine à la position 410, en se référant à la numérotation d'acides aminés des SEQ ID NO : 1, SEQ ID NO : 21 et SEQ ID NO : 22. Selon l'invention, la cellulase présente une ou plusieurs des propriétés suivantes : une thermostabilité accrue, une thermophilie accrue, une alcalophilie accrue ou une combinaison de ces propriétés.

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