Fast photochemical method of labelling nucleic acids for...

Details Fast photochemical method of labelling nucleic acids for... Fast photochemical method of labelling nucleic acids for...

C - Chemistry, Metallurgy

12

Q

204/91.11, 195/1

C12Q 1/68 (2006.01) C12N 15/00 (2006.01) G01N 33/50 (2006.01) G01N 33/52 (2006.01) G01N 33/58 (2006.01)

Patent

CA 1222705

ABSTRACT A labeled nucleic acid probe comprising (a) a nucleic acid component, (b) a nucleic acid-binding ligand photochemically linked to the nucleic acid component, and (c) a label chemically linked to the nucleic acid-binding ligand. The label can be a specifically bindable ligand such as a hapten or biotin, an enzyme such as a .beta.-galactosidase or horse radish peroxidase, a fluorescent radical, a phycobiliprotein, a luminescent radical, or a radioisotope. The probe can be used in assays of nucleic acids, taking advantage of the ability of the nucleic acid component to hybridize.

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