Fatty acid desaturases and mutant sequences thereof

Details Fatty acid desaturases and mutant sequences thereof Fatty acid desaturases and mutant sequences thereof

C - Chemistry, Metallurgy

12

N

C12N 15/53 (2006.01) C07H 21/00 (2006.01) C12N 9/02 (2006.01) A01H 5/00 (2006.01)

Patent

CA 2575962

Seeds, plants and oils are provided having high oleic acid; low linoleic acid; and low linoleic acid plus linolenic acid; and advantageous functional or nutritional properties. Plants are disclosed that contain a mutation in a delta-12 or delta-15 fatty acid desaturase gene. Preferred plants are rapeseed and sunflower plats. Plants carrying such mutant genes have altered fatty acid composition in seeds. In one embodiment, a plant contains a mutation in a region having the conserved motif His-Xaa-Xaa-His, found in delta-12 and delta-15 fatty acid desaturases. A preferred motif has the sequence His-Glu-Cys-Gly-His. A preferred mutation in this motif has the amino acid sequence His-Lys-Cys-Gly-His. Nucleic acid fragments are disclosed that comprise a mutant delta-12 or delta-15 fatty acid desaturase gene sequence.

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