C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/54 (2006.01) A23L 1/30 (2006.01) A23L 1/307 (2006.01) C12N 15/29 (2006.01) C12P 7/64 (2006.01) C12P 21/02 (2006.01)
Patent
CA 2352473
By this invention, nucleic acid sequences encoding for fatty acyl-CoA: fatty alcohol acyltransferase (wax synthase) are provided, wherein said wax synthase is active in the formation of a wax ester from fatty alcohol and fatty acyl- CoA substrates. Of special interest are nucleic acid sequences obtainable from a jojoba embryo wax synthase having an apparent molecular mass of approximately 33kD. Also considered are amino acid and nucleic acid sequences obtainable from wax synthase proteins and the use of such sequences to provide transgenic host cells capable of producing wax esters.
La présente invention concerne des séquences d'acides nucléiques codant pour des acyle gras CoA: alcool gras acyltransférases (cire-synthétase), ladite cire-synthétase étant active dans la formation d'un ester de cire à partir de substrats d'alcools gras et d'acyle gras-CoA. Les séquences nucléiques pouvant être obtenues à partir de la cire-synthétase de germe de jojoba présentant une masse moléculaire apparente d'environ 33 kD sont particulièrement intéressantes. Cette invention concerne également des séquences d'acides aminés et des séquences d'acides nucléiques qui peuvent être obtenues à partir de protéines de cire-synthétase, et l'utilisation de telles séquences aux fins d'obtenir des cellules hôtes transgéniques capables de produire des esters de cire.
Lardizabal Kathryn Dennis
Lassner Michael W.
Metz James George
Calgene Llc
Mcfadden Fincham
LandOfFree
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