Fc labeling for immunostaining and immunotargeting

A - Human Necessities – 61 – K

Patent

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Details

A61K 39/395 (2006.01) C07K 16/28 (2006.01) C07K 16/46 (2006.01) C12P 21/06 (2006.01)

Patent

CA 2630415

The present invention discloses methods of labeling Fc portions of antibodies, or fusion proteins incorporating Fc portions of antibodies, so that they can be used in immunostaining or immunoiabeling procedures. A wide variety of labels can be used. A linker can be used between the label and the protein to be labeled, allowing for flexibility in labeling. A large variety of coupling reactions can be used to generate the labeled protein molecule. The protein molecule to be labeled can be part of a larger fusion protein. The labeled protein molecules can be used in immunostaining and immunolabelÊng procedures but also in in vivo applications for therapy and diagnostic imaging.

La présente invention concerne des procédés de marquage de parties Fc d'anticorps ou de protéines de fusion incorporant des parties Fc d'anticorps, de façon à pouvoir les utiliser dans des procédures d'immunocoloration ou d'immunomarquage. On peut utiliser un grand nombre de marqueurs. On peut utiliser un groupe de liaison entre le marqueur et la protéine à marquer, permettant une flexibilité dans le marquage. On peut utiliser un grand nombre de réactions de couplage pour générer la molécule de protéine marquée. La molécule de protéine à marquer peut être une partie d'une protéine de fusion plus grande. Les molécules de protéines marquées peuvent être utilisées dans des procédures d'immunocoloration et d'immunomarquage mais également dans des applications in vivo pour l'imagerie de thérapie et de diagnostic.

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