Fed batch culture method for protein secreting cells

Details Fed batch culture method for protein secreting cells Fed batch culture method for protein secreting cells

C - Chemistry, Metallurgy

12

N

C12N 15/11 (2006.01) C12N 5/02 (2006.01) C12N 9/64 (2006.01) C12P 21/02 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2111561

2111561 9322448 PCTABS00027 In a process of production of a useful protein by culturing a human embryonal kidney cell-derived 293 strain which is introduced with a useful protein-expressing gene. It becomes possible to obtain a culture fluid containing the useful protein in a high concentration using an extremely small amount of the medium, by carrying out a fed-batch culture and adding a sugar or a sugar and a calcium in a specified stage of the culture.

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