Feeder-free pluripotent stem cell media containing human serum

C - Chemistry – Metallurgy – 12 – N

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C12N 5/0735 (2010.01) C12N 5/02 (2006.01)

Patent

CA 2703116

The present invention provides compositions and methods for the culture and maintenance of pluripotent stem cells. More particularly, the present invention provides for compositions and methods for culturing, maintaining, growing and stabilizing primate pluripotent stem cells in a feeder-free defined media further comprising human serum, or a soluble attachment component of the human serum, for promoting cell attachment.

La présente invention a pour objet des compositions et des procédés pour la culture et la maintenance de cellules souches pluripotentes. Plus particulièrement, la présente invention a pour objet des compositions et des procédés pour la culture, la maintenance, la croissance et la stabilisation de cellules souches pluripotentes de primate dans des milieux définis exempts d'alimentation comprenant en outre du sérum humain, ou un composant de fixation soluble du sérum humain, pour favoriser la fixation des cellules.

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