Feeding assay for identifying nematicidal compounds

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/00 (2006.01) C12Q 1/02 (2006.01) G01N 33/15 (2006.01) G01N 33/50 (2006.01)

Patent

CA 2392540

The invention relates to any assay for the identification of nematicidal compounds such as anthelmintics for the control of nematodes. The assay comprises: (i) incubating a nematode sample (i.e. a "test nematode sample") containing a predetermined amount of nematodes with an ingestable substrate labelled with a readily detectable label, under conditions suitable for nematode feeding, wherein said incubation is conducted in the presence of said candidate nematicidal compound or follows a pre-incubation of said nematode sample with said candidate nematicidal compound, and (ii) determining whether said candidate nematicidal compound affects nematode feeding by detecting an increase or decrease in ingested substrate as compared to that which has been ingested by a control nematode sample which has been incubated without said candidate nematicidal compound.

L'invention concerne un dosage pour identifier des composés nématicides tels que les antihelminthiques destinés à la lutte contre les nématodes. L'utilisation du dosage consiste en ce qui suit: (i) incuber un échantillon de nématode (p.ex., un "dosage de nématodes de test") contenant une quantité prédéterminée de nématodes avec une substrat ingestible marqué avec un étiquette facilement détectable, dans des conditions adaptées à l'alimentation des nématodes, ladite incubation étant conduite en présence dudit composé candidat nématicide ou après une pré-incubation dudit échantillon de nématode avec ce composé candidat nématicide; et (ii) déterminer si ce composé candidat nématicide affecte l'alimentation du nématode par la détection d'une augmentation ou d'une diminution du substrat ingéré en comparaison à celui ingéré par un échantillon de nématode de contrôle qui a été incubé sans ce composé candidat nématicide.

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