Feline infectious peritonitis vaccine and method of preparation

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 7/04 (2006.01) A61K 39/12 (2006.01) A61K 39/215 (2006.01) C07K 14/165 (2006.01) C12N 5/10 (2006.01) A61K 39/00 (2006.01)

Patent

CA 2128698

2128698 9315762 PCTABS00024 A vaccine for the prevention of disease caused by feline infectious peritonitis virus (FIPV) which comprises FIP viral precursor immunogens derived from in vitro produced cells persistently infected with FIPV. A method of producing FIP viral precursor immunogens is disclosed which comprises culturing in vitro FIP-persistently infected cells in a serum-containing growth medium, subsequently transferring and maintaining said cultured cells in a serum-free medium under conditions for a time adequate to accumulate FIP viral precursor immunogens shed from said cells, and then separating the cells from the supernatant containing the viral precursor immunogens. Any live virus present in the mixture is inactivated and the supernatant containing the FIP viral precursor immunogens is blended with a pharmaceutically-acceptable adjuvant in order to form the FIP vaccine disclosed herein.

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