Fen-1 endonucleases from archaeoglobus veneficus

Details Fen-1 endonucleases from archaeoglobus veneficus Fen-1 endonucleases from archaeoglobus veneficus

C - Chemistry, Metallurgy

12

N

C12N 15/55 (2006.01) A61K 31/7088 (2006.01) A61K 38/46 (2006.01) A61K 48/00 (2006.01) C07H 21/00 (2006.01) C12N 1/21 (2006.01) C12N 9/12 (2006.01) C12N 9/22 (2006.01) C12N 15/10 (2006.01) C12P 19/34 (2006.01) C12Q 1/44 (2006.01) C12Q 1/68 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2428798

The present invention provides novel cleavage agents and polymerases for the cleavage and modification of nucleic acid. The cleavage agents and polymerases find use, for example, for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. In some embodiments, the 5' nuclease activity of a variety of enzymes is used to cleave a target- dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.

L'invention concerne des agents de scission et des polymérases destinés à la scission et à la modification d'acide nucléique. Les agents de scission et les polymérases trouvent une utilisation, par exemple, dans la détection et la caractérisation de séquences d'acides nucléiques et de variations dans les séquences d'acides nucléiques. Dans certaines réalisations, on utilise l'activité nucléase 5' d'une variété d'enzymes afin de couper une structure dont la scission potentielle est fonction d'une cible, ce qui permet d'indiquer la présence de séquences spécifiques d'acides nucléiques ou des variations spécifiques de ces séquences.

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