C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 15/55 (2006.01) C12N 1/21 (2006.01) C12N 9/22 (2006.01) C12N 15/09 (2006.01) C12P 19/34 (2006.01) C12Q 1/34 (2006.01) C12Q 1/44 (2006.01) C12Q 1/68 (2006.01)
Patent
CA 2273204
The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to improved cleavage means for the detection and characterization of nucleic acid sequences. Structure-specific nucleases derived from a variety of thermostable organisms are provided. These structure-specific nucleases are used to cleave target-dependent cleavage structures, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
L'invention concerne un système permettant de détecter et de caractériser des séquences d'acide nucléique, ainsi que des variations dans lesdites séquences. Elle concerne également un système de clivage amélioré permettant de détecter et de caractériser des séquences d'acide nucléique. Elle concerne enfin des nucléases spécifiques de structure dérivées de divers organismes thermostables, qui sont utilisées pour couper des structures de clivage dépendantes de la cible, de façon à indiquer la présence de séquences d'acide nucléique spécifiques ou de variations spécifiques desdites séquences.
Kaiser Michael W.
Lyamichev Victor I.
Lyamicheva Natasha
Smart & Biggar
Third Wave Technologies Inc.
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