C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
C12Q 3/00 (2006.01) C07K 14/765 (2006.01) C12M 1/34 (2006.01) C12M 1/36 (2006.01) C12N 1/14 (2006.01) C12N 1/20 (2006.01) C12P 21/02 (2006.01)
Patent
CA 2242323
A process of culturing a microorganism in a culture medium in which process the addition of feed medium is controlled by using the production of a by- product as a measure of the culture conditions, characterised in that the by- product is an electrically charged metabolite produced by the microorganism, and in that the production of the metabolite is monitored by measuring the conductance of the culture medium. The metabolite may be acetate and the microorganism may be yeast which is genetically engineered to produce a desired polypeptide.
L'invention concerne un procédé de mise en culture d'un micro-organisme dans un milieu de culture, procédé dans lequel on régule l'ajout d'un milieu d'apport par la production d'un sous-produit comme mesure des conditions de culture. Ce procédé se caractérise en ce que le sous-produit est un métabolite chargé électriquement produit par le micro-organisme, et que la production du métabolite est contrôlée par la mesure de la conductance dans le milieu de culture. Le métabolite peut être un acétate et le micro-organisme une levure issue du génie génétique pour produire un polypeptide désiré.
Mead David John
Van Urk Hendrik
Bereskin & Parr Llp/s.e.n.c.r.l.,s.r.l.
Delta Biotechnology Limited
Novozymes Delta Limited
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