C - Chemistry – Metallurgy – 12 – M
Patent
C - Chemistry, Metallurgy
12
M
C12M 1/24 (2006.01) C12M 1/02 (2006.01) C12M 1/12 (2006.01) C12N 1/00 (2006.01) C12N 5/02 (2006.01)
Patent
CA 2509733
A container is provided for cell growth by artificial cultivation of selected biological material in a growth media for use in an oscillating incubator. The container has a circular bottom with rounded corners joining a sidewall that is inclined at an angle toward a longitudinal axis of the container to define a container volume. The bottom has six equally spaced baffles extending inward from the corners and upward toward a large diameter opening of the container centered on the longitudinal axis. The baffles have a triangular cross-sectional shape with an included angle of about 28-40°, and a height measured parallel to the longitudinal axis of about 15-25% of the usable container volume. The baffles end before the longitudinal axis. The container and baffles are blow molded from a polymer suitable for blow molding. An air permeable filter is placed over the container opening, with the filter having an adhesive on one side to stick to the container. A finger tab on the periphery of the filter helps to position and remove the filter. A growth media is placed in the container along with a biological material to be cultured and rotated from about 250RPM to 450RPM depending on the volume of the material and the container. The growth media comprises MgBr2, CuBr2, FeBr2, Tris, Tris-HBr, Tris-acetate, or Tris-HCl, KBr, adenine, palmitic acid, glycerol or D-(+)-glucose, or both, ATP, Protein Hydrolysate Amicase or other casamino acids, yeast or yeast extract, tryptone peptone, anti-foam and de-ionized water.
Ellis Samuel A.
Harlan Jeffrey L.
Gowling Lafleur Henderson Llp
Scientific Plastic Products Inc.
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