C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
195/43, 195/51
C12N 1/19 (2006.01) C07K 14/02 (2006.01) C12N 1/18 (2006.01)
Patent
CA 1288075
Abstract A method for culturing yeast capable of expressing an exogenous gene product wherein the yeast is cultured at about 25°C in a medium including balanced free amino acids at a concentration between about 20 g/l and about 160 g/l. Alternatively, yeast capable of expressing an exogenous gene product (e.g. the product of a gene introduced by the application of recombinant gene technology) may be introduced into a medium including a total carbon source concentration above but maintained as close to zero as possible, and cultured at about 25°C. A medium for fermentation of yeast capable of expressing an exogenous gene product includes balanced free amino acids at a concentration between about 20 g/l and about 160 g/l. The culture medium is preferably saturated with oxygen at least 30% of air saturation.
516108
Amgen Inc.
Ridout & Maybee Llp
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