B - Operations – Transporting – 05 – B
Patent
B - Operations, Transporting
05
B
B05B 12/00 (2006.01) B05B 1/18 (2006.01) G05D 9/00 (2006.01) G05D 21/02 (2006.01)
Patent
CA 2376515
A fermentation process post-sterile additive device and method of operation is detailed. The device is used to deliver additive as a heavy-drop mist. The mist covers the entire fermenting batch head surface of the vat broth. This improves mixing and reduces the amount of additive to appropriate introduction levels throughout log phase fermentation, preventing coating of cultivating cells and interference with cell respiration. A steady state growth rate is fostered.
La présente invention concerne un dispositif d'introduction d'additif stérile en fermentation et son procédé de mise en oeuvre. Ce dispositif est utilisé afin d'introduire un additif sous forme de brouillard dense. Le brouillard couvre toute la surface supérieure du lot en fermentation du milieu de culture contenu dans le bioréacteur. Selon l'invention, le mélange est amélioré et la quantité d'additif est réduite à des niveaux d'introduction appropriés tout au long de la phase logarithmique de fermentation, ce qui permet d'éviter un revêtement des cellules et d'interférer avec leur respiration. On favorise ainsi un taux de croissance stationnaire.
Icos Corporation
Smart & Biggar
LandOfFree
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