Fermentation process

C - Chemistry – Metallurgy – 12 – P

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C12P 7/62 (2006.01) C07D 307/33 (2006.01) C12P 7/40 (2006.01) C12P 17/04 (2006.01)

Patent

CA 1108078

ABSTRACT OF THE DISCLOSURE The invention concerns a fermentation process for the production of (+)-2(5.beta.-n-butyl-4.beta.-hydroxy-2-oxo- tetrahydrofuran-3.beta.-yl)-2.alpha.-methylacetic acid, which compound has useful ulcer-healing properties. The process is characterised by cultivating an organism known to produce dihydrocanadensolide, such as Penicillium canadense, under generally conventional conditions and then extracting the culture filtrate at a pH in the range pH 3.0-7.0 with a suitable solvent, such as ethyl acetate, and then evaporating the extract to dryness. In a preferred embodiment the extraction is carried out on the culture filtrate at pH 4.0- 4.5.

294038

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